GM Food Testing Results!

 After applying the DNA of our sample foods into the gel and using gel electrophoresis, we wait to see the results. We find that the orange we tested was in fact a GM food.

pGLO results!!

The end results took a while to incubate, however our results were great when the bacteria finally matured.

Green florescent success!!

GM Testing Introduction!!

GMO stands for Genetically Modified Organisms. GMOs are forbidden in some European countries, but widely sold in the United States of America. One popular class of GM crops has a gene from the soil bacterium Bacillus Thuringiensis (Bt), put in the borers, common pests on corn. Farmers that plant Bt crops do not have to apply the pesticides because the plants are producing toxic proteins in their cells. GMOs can also include crops with herbicide-resistant delayed for fruit ripening, fungi resistant, drought resistant, have increased crop count, and/or bear improved crops. The controversy is that the food may improve nutrition, may create a surplus of food, and may helped meds/drugs. However, there may be food allergies to the chemicals, monoculture, and uproar about tinkering with evolution.
In this lab, we will be checking certain foods to see if they are GM foods. HYPOTHSIS: I think most of the food today will be Gm foods.

pGLO transformation lab Introduction!!

A gene is a piece of DNA which provides the instructions for making (codes for) a protein. A genetic transformation means change by genes, and involves the insertion of a gene into an organism in order to change the organism's trait. Genetic transformation is used in many areas of biotechnology such as agriculture, bioremediation, and medicine. In this lab we will transform bacteria with a gene that codes for Green Fluorescent Protein (GFP). These genes will come from a live Sea Jelly (bioluminescent jellyfish Aequorea victoria. A green fluorescent protein causes the jellyfish to fluoresce and glow in dim light or in pitch black depths of the ocean. The bacteria that might aquire this green fluorescent protein might glow under ultraviolet light. HYPOTHESIS: I think the bacteria with the newly aquired green fluorescent protien will glow under ultraviolet light.

Introduction

Lab: DNA Chips: Genes to Disease
Using Microarrays to Study Genes Involved in Lung Cancer

Background: Microarray help to accelerate scientists in their research. This enables scientists to look at more then one type of cell or cells. A DNA chip, microarray, gene chip, and genome chip are all terms that describe a solid matrix, like a glass slide, that has arranged spots, each a copy of a specific oligonucleotide representing part of a genome. Analysis of a DNA chip shows genes that were transcribed in the cancerous tissue and which in the normal tissue, and indicates which genes might be important in the development of cancer. One microarray can contain more than 30,000 spots of DNA, each representing a different gene in an organism.

Discussion!

When we got the finished results from the gel, the results showed that the person responsible was suspect 2 aka Katie Records. Her DNA matched the DNA from the crime scene. The only mistake we might have made was not going deep enough in the gel while injecting the DNA.

Introduction!

Technicians in forensic labs are usually asked to do DNA profiling or "fingerprinting" to help or analyze a case for law enforcement or other uses. DNA profiling is a technology based on detecting differences in the repeat copy number. Patterns of produced samples represent DNA taken at the scene of the crime. The restriction enzyme can be used to cut DNA isolated from any source. Agarose gel electrophoresis separates DNA fragments by size. The evidence needed for DNA fingerprinting can be obtained by biological material such as: body tissues, body fluids (blood and semen), hair follicles, even dried blood stains or mummified tissues. The restriction enzyme works best under a specific buffer and temperature conditions. In our lab- CSI: AHS or 94549; we will be using the restriction enzymes to cut DNA palindromes. RFLP stands for Restriction Fragment Length Polymorphism. We will cut DNA and generate fragments of different length-"run a gel"; crime scene-"run to red"; DNA from suspects.